This content of caffeic acid,salvianolic acid E,rosmarinic acid,lithospermic acid,salvianolic acid B,and salvianolic acid Y in the salvianolic acids for shot was also determined. The linear relationship between chemicals had been great( r>0. 998 9),and the injection accuracy RSD had been 0. 30%-0. 90%. The sexual RSD is between 1. 4% and 3. 0%,and the RSD of the reproducibility regarding the herb is between 2. 1% and 5. 2%. The recovery rate associated with three elements in S. miltiorrhiza had been 96. 80%-99. 20%,and the recovery price associated with influence of mass media six components in salvianolic acids for shot was 88. 90%-107. 5%. The perfect solution is of S. miltiorrhiza and salvianolic acids for shot were steady within 48 h. A complete of 8 batches of S. miltiorrhiza and injection were dependant on the reference plant,and the real difference was smaller than that measured by the monomer control. This research preliminarily validated that the salvianolic acids reference extract can be used as an alternative for the monomer control for the quality-control of S. miltiorrhiza and salvianolic acids for injection.This study is designed to establish a method when it comes to dedication of As B,As C,DMA,As( Ⅲ),MMA and As( Ⅴ) using HPLC-ICP-MS. A Dioncx Ion PacTMAS7( 4 mm×250 mm) column was used for the HPLC-ICP-MS technique. The cellular period had been 100 mmol·L-1 ammonium carbonate-1. 5 mmol·L-1 ammonium dibasic phosphate( gradient elution) at a flow price of 1 m L·min-1. The shot amount was 10 μL. The linear relationships of As B,As C,DMA,As( Ⅲ),MMA,As( Ⅴ) had been good with all the concentration of10-500 μg·L-1. The common recovery rates( letter = 6) were 105. 7%,100. 5%,102. 9%,105. 7%,100. 2%,92. 69%. The RSD were0. 50%,2. 4%,0. 93%,1. 3%,0. 89%,1. 5%. The precision and repeatability for this strategy had been great. In this study,six kinds of arsenic were separated effectively by this technique. With methodological validation and sample dedication,this method can be used to AHPN agonist cell line figure out the morphological valence of arsenic in content determination.Artemisiae Argyi Folium,the dried leaves of Artemisia argyi,has been commonly utilized in traditional Chinese and people drugs for a long period. Qiai is among the top-geoherb of Artemisiae Argyi Folium. Attempting to research dynamic changes of chemical components of Qiai in numerous harvest times and explore the optimum collect period of Qiai,in this study,the contents of complete flavonoids and total phenolic acids of 36 batches of Qiai gathered in 6 different collect times were analyzed by ultraviolet-visible spectrophotometry. Furthermore,an HPLC method was requested multiple determination of eight bioactive compounds including six phenolic acids( 5-caffeoylquinic acid,3-caffeoylquinic acid,4-caffeoylquinic acid,3,4-di-O-caffeoylquinic acid,3,5-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid) and two flavonoids( jaceosidin and eupatilin) in Qiai samples. The quantitative outcomes suggested that there were some differences in the items of total flavonoids,total phenolic acids and bioactive substances of Qiai examples in numerous collect periods. The dynamic modifications of complete flavonoids and total phenolic acids of Qiai in numerous harvest durations were constant. The contents of complete flavonoids and complete phenolic acids of Qiai samples were higher into the third harvest duration( across the Dragon Boat Festival),which is fundamentally in line with the original harvest periods. This current research provides the basis for identifying Medicina defensiva the proper harvest time of Qiai,and may be useful for the high quality assessment of the herbal medicine.This research had been carried out to review the additional metabolites of endophytic fungus Aspergillosis fumigatus from Euphorbia royleana. The endophytic fungus A. fumigatus was fermented by solid fermentation,and purified by numerous chromatographic practices after removal. The structures associated with the compounds had been identified by1 H-NMR,13 C-NMR and HSQC,HMBC spectra and physicchemical properties. Three substances were separated and their particular frameworks were defined as 3-( 3,4-dihydroxybenzoyl)-5-( 3,4-dihydroxyphenyl)-6-methyl-5,6-dihydro-2 H-pyran-2-one( 1),hydroxysydonic acid( 2) and 11-hydroxysydonic acid( 3). Compound 1 is a unique compound.By preparing 10 batches regarding the content reference of Linggui Zhugan Decoction,the methodology of the characteristic spectral range of the material guide was made. The creaming rate range,the contents together with transfer rate number of cinnamaldehyde,glycyrrhizin and glycyrrhizic acid,the characteristic peaks in addition to similarity range of the characteristic spectral range of Linggui Zhugan Decoction were determined to make clear crucial high quality attributes associated with content reference of Linggui Zhugan Decoction. When you look at the 10 batches of this material reference of Linggui Zhugan Decoction,the similarity of characteristic spectrum was higher than 0. 9. Furthermore,after summarizing the characteristic top information,we understood that Fuling had two characteristic peaks,Guizhi had six characteristic peaks,Baizhu had two characteristic peaks and Gancao had 11 characteristic peaks. The typical creaming price of this content reference of this ten batches had been( 12. 13 ± 0. 35) %. The typical content of cinnamaldehyde was 0. 32%,the average transfer price was 10. 69%,the content of cinnamaldehyde when you look at the different batches had been between 0. 22% and 0. 42%,and the transfer price had been between 7. 48% and13. 90%. The average content of glycyrrhizin was 0. 84%,the typical transfer rate had been 50. 39%,the content of glycyrrhizin into the different batches ended up being between 0. 42% and 1. 26%,and the transfer price ended up being between 35. 27% and 65. 51%. The common content of glycyrrhizic ended up being 1. 88%,the average transfer price was 40. 74%,the content of glycyrrhizic into the various batches had been between 0. 94% and2. 82%,and the transfer price was between 28. 52% and 52. 96%. In this paper,the quality value transmitting of compound benchmarks of Linggui Zhugan Decoction ended up being reviewed by the combination of characteristic spectrum,creaming price together with content of list element.
Categories